Genome-wide BAC-end sequencing of Cucumis melo using two BAC libraries
نویسندگان
چکیده
منابع مشابه
High throughput direct end sequencing of BAC clones.
Libraries constructed in bacterial artificial chromosome (BAC) vectors have become the choice for clone sets in high throughput genomic sequencing projects primarily because of their high stability. BAC libraries have been proposed as a source for minimally over-lapping clones for sequencing large genomic regions, and the use of BAC end sequences (i.e. sequences adjoining the insert sites) has ...
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The majority of gene-targeting experiments in mice are performed in 129Sv-derived embryonic stem (ES) cell lines, which are generally considered to be more reliable at colonizing the germ line than ES cells derived from other strains. Gene targeting is reliant on homologous recombination of a targeting vector with the host ES cell genome. The efficiency of recombination is affected by many fact...
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Dinoflagellates are ubiquitous marine and freshwater protists. As free-living photosynthetic plankton, they account for ~50% of the primary productivity of oceans and lakes. As photosynthetic symbionts, they provide essential nutrients to most corals and numerous other marine invertebrates, supporting coral reefs, one of the most diverse ecosystems on earth, a rich food source, and a potential ...
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Feeding damage caused by the western corn rootworm, Diabrotica virgifera virgifera, is destructive to corn plants in North America and Europe where control remains challenging due to evolution of resistance to chemical and transgenic toxins. A BAC library, DvvBAC1, containing 109,486 clones with 104 ± 34.5 kb inserts was created, which has an ~4.56X genome coverage based upon a 2.58 Gb (2.80 pg...
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ژورنال
عنوان ژورنال: BMC Genomics
سال: 2010
ISSN: 1471-2164
DOI: 10.1186/1471-2164-11-618